MONOCLONAL ANTI-6X HIS TAG

Application

Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.Monoclonal Anti-6X His tag antibody produced in mouse has been used in Co-Immunoprecipitation (Co-IP) and immunoblotting.

Biochem/physiol Actions

6X His tag does not affect the structure and function of protein and can be used in antibody production. It is also used to purify proteins under native or denaturing form.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

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General description

6X His is a histidine tag that consists of a series of histidine residues at the amino or carboxy terminus of a protein.

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Other Notes

Purification: Affinity purified by Protein G

Physical form

Phosphate-buffered saline, no preservative added.